Hyper Stacks 1994 May

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/ Hyper Stacks 1994 May / Hyper Stacks (Pacific HiTech)(1994)[Mac].iso / Science / Biology / RefStack Folder / RNase P refs 2.0 < prev
MacBinary | 1990-01-05 | 173.3 KB | [STAK/WILD]
open in:
MacOS 8.1 extracted |
Win98 extracted |
DOS extracted
view JSON data |
view as text

This file was processed as: MacBinary (archive/macBinary).

You can browse this item here: RNase P refs 2.0
Confidence	Program	Detection	Match Type	Support
`100%`	dexvert	HyperCard Stack `(archive/hypercard)`	idMeta	Supported
`10%`	dexvert	MacBinary `(archive/macBinary)`	fallback	Supported
`1%`	dexvert	PCP archive log `(other/pcpArchiveLog)`	ext	Unsupported
`100%`	file	MacBinary II, inited, Fri Apr 21 07:05:33 1989, modified Fri Jan 5 17:41:05 1990, creator HyperCard, type HyperCard stack, 166784 bytes "RNase P refs 2.0" , at 0x28c00 10397 bytes resource	default (weak)
`99%`	file	data	default
`74%`	TrID	Mac HyperCard stack (MacBinary)	default
`25%`	TrID	MacBinary 2	default (weak)
`100%`	lsar	MacBinary	default
id metadata
key	value
macFileType	`[STAK]`
macFileCreator	`[WILD]`
hex view
+--------+-------------------------+-------------------------+--------+--------+
|00000000| 00 10 52 4e 61 73 65 20 | 50 20 72 65 66 73 20 32 |..RNase |P refs 2|
|00000010| 2e 30 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |.0......|........|
|00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000040| 00 53 54 41 4b 57 49 4c | 44 01 00 00 00 00 00 00 |.STAKWIL|D.......|
|00000050| 00 00 00 00 02 8b 80 00 | 00 28 9d a0 74 bb 7d a1 |........|.(..t.}.|
|00000060| ca d3 01 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 73 c4 00 00 |........|....s...|
|00000080| 00 00 12 00 53 54 41 4b | ff ff ff ff fa 22 41 0f |....STAK|....."A.|
|00000090| 00 00 00 08 00 02 8b 80 | 00 00 12 00 00 00 00 00 |........|........|
|000000a0| 00 00 00 01 00 00 00 01 | 00 00 0a 96 00 00 00 75 |........|.......u|
|000000b0| 00 00 74 4f 00 00 45 bc | 00 00 00 00 00 00 00 00 |..tO..E.|........|
|000000c0| 00 00 0d f7 00 00 00 00 | 00 05 00 00 10 00 00 00 |........|........|
|000000d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000000e0| 00 00 00 00 01 22 80 00 | 01 22 80 00 01 22 80 00 |....."..|."..."..|
|000000f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000100| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000110| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000120| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000130| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000140| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000150| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000160| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000170| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000180| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000190| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000200| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000210| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000220| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000230| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000240| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000250| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000260| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000270| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000280| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000290| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000300| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000310| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000320| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000330| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000340| 00 00 00 00 00 00 00 00 | 00 00 22 00 00 00 22 00 |........|.."...".|
|00000350| 88 00 22 00 88 00 22 00 | cc 00 33 00 cc 00 33 00 |.."...".|..3...3.|
|00000360| cc 88 33 22 cc 88 33 22 | ee 88 bb 22 ee 88 bb 22 |..3"..3"|..."..."|
|00000370| ee cc bb 33 ee cc bb 33 | ff cc ff 33 ff cc ff 33 |...3...3|...3...3|
|00000380| ff ee ff bb ff ee ff bb | ff ff ff bb ff ff ff bb |........|........|
|00000390| 80 10 02 20 01 08 40 04 | ff ff ff ff ff ff ff ff |... ..@.|........|
|000003a0| 88 22 88 22 88 22 88 22 | 11 22 44 88 11 22 44 88 |."."."."|."D.."D.|
|000003b0| c4 80 0c 68 43 02 30 26 | b1 30 03 1b d8 c0 0c 8d |...hC.0&|.0......|
|000003c0| aa 00 aa 00 aa 00 aa 00 | 88 22 55 22 88 22 55 22 |........|."U"."U"|
|000003d0| 88 55 22 55 88 55 22 55 | 77 dd 77 dd 77 dd 77 dd |.U"U.U"U|w.w.w.w.|
|000003e0| 80 00 00 00 00 00 00 00 | aa 55 aa 55 aa 55 aa 55 |........|.U.U.U.U|
|000003f0| 03 84 48 30 0c 02 01 01 | 82 44 39 44 82 01 01 01 |..H0....|.D9D....|
|00000400| 88 14 22 41 88 41 22 14 | 80 80 41 3e 08 08 14 e3 |.."A.A".|..A>....|
|00000410| 22 04 8c 74 22 17 98 10 | be 80 88 08 eb 08 88 80 |"..t"...|........|
|00000420| 25 c8 32 89 64 24 4c 92 | a2 9c 41 be 2a c9 14 eb |%.2.d$L.|..A.*...|
|00000430| 40 a0 00 00 04 0a 00 00 | 80 40 20 00 02 04 08 00 |@.......|.@ .....|
|00000440| aa 00 80 00 88 00 80 00 | ff 80 80 80 80 80 80 80 |........|........|
|00000450| 08 1c 22 c1 80 01 02 04 | ff 80 80 80 ff 08 08 08 |..".....|........|
|00000460| f8 74 22 47 8f 17 22 71 | bf 00 bf bf b0 b0 b0 b0 |.t"G.."q|........|
|00000470| ff 7f be 5d a2 41 80 00 | fa f5 fa f5 a0 50 a0 50 |...].A..|.....P.P|
|00000480| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000490| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000500| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000510| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000520| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000530| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000540| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000550| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000560| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000570| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000580| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000590| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000600| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000610| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000620| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000630| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000640| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000650| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000660| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000670| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000680| 2d 2d 53 63 72 69 70 74 | 73 20 66 6f 72 20 52 45 |--Script|s for RE|
|00000690| 46 45 52 45 4e 43 45 53 | 20 73 74 61 63 6b 0d 2d |FERENCES| stack.-|
|000006a0| 2d 38 2f 32 30 2f 38 39 | 20 62 79 20 4a 57 42 72 |-8/20/89| by JWBr|
|000006b0| 6f 77 6e 0d 0d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |own..---|--------|
|000006c0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006d0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006e0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006f0| 0d 2d 2d 20 4d 65 6e 75 | 62 61 72 20 75 6e 69 74 |.-- Menu|bar unit|
|00000700| 0d 0d 6f 6e 20 6f 70 65 | 6e 53 74 61 63 6b 0d 67 |..on ope|nStack.g|
|00000710| 6c 6f 62 61 6c 20 4d 45 | 4e 55 0d 70 75 74 20 4e |lobal ME|NU.put N|
|00000720| 65 77 4d 65 6e 75 28 22 | 52 65 66 65 72 65 6e 63 |ewMenu("|Referenc|
|00000730| 65 73 22 2c 20 22 41 62 | 6f 75 74 20 52 65 66 65 |es", "Ab|out Refe|
|00000740| 72 65 6e 63 65 73 2e 2e | 2e 22 2c 22 28 2d 22 2c |rences..|.","(-",|
|00000750| 22 52 65 61 64 20 54 65 | 78 74 20 66 69 6c 65 2e |"Read Te|xt file.|
|00000760| 2e 2e 22 2c 22 43 72 65 | 61 74 65 20 54 65 78 74 |..","Cre|ate Text|
|00000770| 20 66 69 6c 65 2e 2e 2e | 22 2c 22 28 2d 22 2c 22 | file...|","(-","|
|00000780| 50 72 69 6e 74 20 72 65 | 66 65 72 65 6e 63 65 22 |Print re|ference"|
|00000790| 2c 22 50 72 69 6e 74 20 | 41 4c 4c 20 72 65 66 73 |,"Print |ALL refs|
|000007a0| 2e 2e 2e 22 29 20 69 6e | 74 6f 20 4d 45 4e 55 0d |...") in|to MENU.|
|000007b0| 45 6e 61 62 6c 65 4d 65 | 6e 75 20 4d 45 4e 55 2c |EnableMe|nu MENU,|
|000007c0| 32 2c 46 41 4c 53 45 0d | 45 6e 61 62 6c 65 4d 65 |2,FALSE.|EnableMe|
|000007d0| 6e 75 20 4d 45 4e 55 2c | 35 2c 46 41 4c 53 45 0d |nu MENU,|5,FALSE.|
|000007e0| 65 6e 64 20 6f 70 65 6e | 73 74 61 63 6b 0d 0d 6f |end open|stack..o|
|000007f0| 6e 20 63 6c 6f 73 65 53 | 74 61 63 6b 0d 67 6c 6f |n closeS|tack.glo|
|00000800| 62 61 6c 20 4d 45 4e 55 | 0d 70 75 74 20 44 65 6c |bal MENU|.put Del|
|00000810| 65 74 65 4d 65 6e 75 28 | 4d 45 4e 55 29 20 69 6e |eteMenu(|MENU) in|
|00000820| 74 6f 20 4d 45 4e 55 0d | 65 6e 64 20 63 6c 6f 73 |to MENU.|end clos|
|00000830| 65 53 74 61 63 6b 0d 0d | 6f 6e 20 69 64 6c 65 0d |eStack..|on idle.|
|00000840| 67 6c 6f 62 61 6c 20 4d | 45 4e 55 2c 4c 41 53 54 |global M|ENU,LAST|
|00000850| 54 49 43 4b 0d 69 66 20 | 28 74 68 65 20 74 69 63 |TICK.if |(the tic|
|00000860| 6b 73 20 2d 20 4c 41 53 | 54 54 49 43 4b 29 3e 31 |ks - LAS|TTICK)>1|
|00000870| 32 30 20 74 68 65 6e 0d | 70 75 74 20 74 68 65 20 |20 then.|put the |
|00000880| 74 69 63 6b 73 20 69 6e | 74 6f 20 4c 41 53 54 54 |ticks in|to LASTT|
|00000890| 49 43 4b 0d 53 68 6f 77 | 4d 65 6e 75 28 4d 45 4e |ICK.Show|Menu(MEN|
|000008a0| 55 29 0d 68 69 64 65 20 | 62 61 63 6b 67 72 6f 75 |U).hide |backgrou|
|000008b0| 6e 64 20 66 69 65 6c 64 | 20 22 68 69 64 64 65 6e |nd field| "hidden|
|000008c0| 66 69 65 6c 64 22 0d 65 | 6e 64 20 69 66 0d 70 61 |field".e|nd if.pa|
|000008d0| 73 73 20 69 64 6c 65 0d | 65 6e 64 20 69 64 6c 65 |ss idle.|end idle|
|000008e0| 0d 0d 6f 6e 20 64 6f 4d | 65 6e 75 20 57 48 49 43 |..on doM|enu WHIC|
|000008f0| 48 0d 69 66 20 57 48 49 | 43 48 20 69 73 20 22 41 |H.if WHI|CH is "A|
|00000900| 62 6f 75 74 20 52 65 66 | 65 72 65 6e 63 65 73 2e |bout Ref|erences.|
|00000910| 2e 2e 22 20 74 68 65 6e | 20 61 62 6f 75 74 0d 65 |.." then| about.e|
|00000920| 6c 73 65 20 69 66 20 57 | 48 49 43 48 20 69 73 20 |lse if W|HICH is |
|00000930| 22 52 65 61 64 20 74 65 | 78 74 20 66 69 6c 65 2e |"Read te|xt file.|
|00000940| 2e 2e 22 20 74 68 65 6e | 20 69 6d 70 6f 72 74 54 |.." then| importT|
|00000950| 65 78 74 0d 65 6c 73 65 | 20 69 66 20 57 48 49 43 |ext.else| if WHIC|
|00000960| 48 20 69 73 20 22 43 72 | 65 61 74 65 20 74 65 78 |H is "Cr|eate tex|
|00000970| 74 20 66 69 6c 65 2e 2e | 2e 22 20 74 68 65 6e 20 |t file..|." then |
|00000980| 63 72 65 61 74 65 54 65 | 78 74 0d 65 6c 73 65 20 |createTe|xt.else |
|00000990| 69 66 20 57 48 49 43 48 | 20 69 73 20 22 50 72 69 |if WHICH| is "Pri|
|000009a0| 6e 74 20 72 65 66 65 72 | 65 6e 63 65 22 20 74 68 |nt refer|ence" th|
|000009b0| 65 6e 20 70 72 69 6e 74 | 4f 75 74 0d 65 6c 73 65 |en print|Out.else|
|000009c0| 20 69 66 20 57 48 49 43 | 48 20 69 73 20 22 50 72 | if WHIC|H is "Pr|
|000009d0| 69 6e 74 20 61 6c 6c 20 | 72 65 66 73 2e 2e 2e 22 |int all |refs..."|
|000009e0| 20 74 68 65 6e 20 70 72 | 69 6e 74 41 6c 6c 0d 65 | then pr|intAll.e|
|000009f0| 6c 73 65 20 70 61 73 73 | 20 64 6f 4d 65 6e 75 0d |lse pass| doMenu.|
|00000a00| 65 6e 64 20 64 6f 4d 65 | 6e 75 0d 0d 2d 2d 2d 2d |end doMe|nu..----|
|00000a10| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000a20| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000a30| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000a40| 2d 2d 2d 2d 2d 2d 2d 0d | 2d 2d 20 46 75 6e 63 74 |-------.|-- Funct|
|00000a50| 69 6f 6e 73 20 75 6e 69 | 74 0d 0d 6f 6e 20 61 62 |ions uni|t..on ab|
|00000a60| 6f 75 74 0d 70 75 74 20 | 74 68 65 20 73 68 6f 72 |out.put |the shor|
|00000a70| 74 20 6e 61 6d 65 20 6f | 66 20 74 68 69 73 20 73 |t name o|f this s|
|00000a80| 74 61 63 6b 20 26 20 22 | 20 53 74 61 63 6b 22 20 |tack & "| Stack" |
|00000a90| 26 20 52 45 54 55 52 4e | 20 69 6e 74 6f 20 54 58 |& RETURN| into TX|
|00000aa0| 54 0d 70 75 74 20 22 2a | 2a 2a 2a 2a 2a 2a 2a 2a |T.put "*|********|
|00000ab0| 2a 2a 2a 2a 2a 2a 2a 2a | 2a 2a 2a 2a 2a 2a 2a 2a |********|********|
|00000ac0| 2a 2a 2a 2a 2a 2a 2a 2a | 2a 22 20 26 20 52 45 54 |********|*" & RET|
|00000ad0| 55 52 4e 20 61 66 74 65 | 72 20 54 58 54 0d 70 75 |URN afte|r TXT.pu|
|00000ae0| 74 20 22 4a 61 6d 65 73 | 20 57 2e 20 42 72 6f 77 |t "James| W. Brow|
|00000af0| 6e 2c 20 49 6e 64 69 61 | 6e 61 20 55 6e 69 76 65 |n, India|na Unive|
|00000b00| 72 73 69 74 79 22 20 26 | 20 52 45 54 55 52 4e 20 |rsity" &| RETURN |
|00000b10| 61 66 74 65 72 20 54 58 | 54 0d 70 75 74 20 22 42 |after TX|T.put "B|
|00000b20| 69 74 4e 65 74 20 61 64 | 64 72 65 73 73 20 3a 20 |itNet ad|dress : |
|00000b30| 42 52 4f 57 4e 4a 57 40 | 49 55 42 41 43 53 22 20 |BROWNJW@|IUBACS" |
|00000b40| 61 66 74 65 72 20 54 58 | 54 0d 61 6c 65 72 74 20 |after TX|T.alert |
|00000b50| 31 2c 31 2c 54 58 54 2c | 30 0d 65 6e 64 20 61 62 |1,1,TXT,|0.end ab|
|00000b60| 6f 75 74 0d 0d 6f 6e 20 | 69 6d 70 6f 72 74 54 65 |out..on |importTe|
|00000b70| 78 74 0d 67 65 74 20 66 | 69 6c 65 4e 61 6d 65 28 |xt.get f|ileName(|
|00000b80| 22 54 45 58 54 22 29 0d | 69 66 20 69 74 20 69 73 |"TEXT").|if it is|
|00000b90| 20 65 6d 70 74 79 20 74 | 68 65 6e 20 65 78 69 74 | empty t|hen exit|
|00000ba0| 20 69 6d 70 6f 72 74 54 | 65 78 74 0d 70 75 74 20 | importT|ext.put |
|00000bb0| 69 74 20 69 6e 74 6f 20 | 46 49 4c 45 50 41 54 48 |it into |FILEPATH|
|00000bc0| 0d 73 65 74 20 63 75 72 | 73 6f 72 20 74 6f 20 34 |.set cur|sor to 4|
|00000bd0| 0d 6f 70 65 6e 20 66 69 | 6c 65 20 46 49 4c 45 50 |.open fi|le FILEP|
|00000be0| 41 54 48 0d 72 65 61 64 | 20 66 72 6f 6d 20 66 69 |ATH.read| from fi|
|00000bf0| 6c 65 20 46 49 4c 45 50 | 41 54 48 20 66 6f 72 20 |le FILEP|ATH for |
|00000c00| 31 36 33 38 34 0d 69 66 | 20 69 74 20 69 73 20 65 |16384.if| it is e|
|00000c10| 6d 70 74 79 20 74 68 65 | 6e 0d 61 6e 73 77 65 72 |mpty the|n.answer|
|00000c20| 20 22 54 68 69 73 20 64 | 69 73 6b 20 66 69 6c 65 | "This d|isk file|
|00000c30| 20 69 73 20 65 6d 70 74 | 79 20 6f 72 20 6e 6f 74 | is empt|y or not|
|00000c40| 20 76 61 6c 69 64 2e 22 | 20 77 69 74 68 20 22 4f | valid."| with "O|
|00000c50| 4f 50 53 21 22 0d 65 78 | 69 74 20 69 6d 70 6f 72 |OPS!".ex|it impor|
|00000c60| 74 54 65 78 74 0d 65 6e | 64 20 69 66 0d 63 6c 6f |tText.en|d if.clo|
|00000c70| 73 65 20 66 69 6c 65 20 | 46 49 4c 45 50 41 54 48 |se file |FILEPATH|
|00000c80| 0d 70 75 74 20 52 45 54 | 55 52 4e 20 61 66 74 65 |.put RET|URN afte|
|00000c90| 72 20 69 74 0d 64 6f 4d | 65 6e 75 20 22 6e 65 77 |r it.doM|enu "new|
|00000ca0| 20 63 61 72 64 22 0d 70 | 75 74 20 69 74 20 69 6e | card".p|ut it in|
|00000cb0| 74 6f 20 62 61 63 6b 67 | 72 6f 75 6e 64 20 66 69 |to backg|round fi|
|00000cc0| 65 6c 64 20 22 63 69 74 | 61 74 69 6f 6e 22 0d 65 |eld "cit|ation".e|
|00000cd0| 6e 64 20 69 6d 70 6f 72 | 74 54 65 78 74 0d 0d 6f |nd impor|tText..o|
|00000ce0| 6e 20 63 72 65 61 74 65 | 54 65 78 74 0d 70 75 74 |n create|Text.put|
|00000cf0| 20 4e 65 77 46 69 6c 65 | 4e 61 6d 65 20 28 22 53 | NewFile|Name ("S|
|00000d00| 61 76 65 20 75 6e 64 65 | 72 20 77 68 61 74 20 6e |ave unde|r what n|
|00000d10| 61 6d 65 3f 22 2c 22 22 | 29 20 69 6e 74 6f 20 69 |ame?",""|) into i|
|00000d20| 74 0d 69 66 20 69 74 20 | 69 73 20 65 6d 70 74 79 |t.if it |is empty|
|00000d30| 20 74 68 65 6e 20 65 78 | 69 74 20 73 61 76 65 54 | then ex|it saveT|
|00000d40| 6f 44 69 73 6b 0d 73 65 | 74 20 74 68 65 20 63 75 |oDisk.se|t the cu|
|00000d50| 72 73 6f 72 20 74 6f 20 | 34 0d 70 75 74 20 69 74 |rsor to |4.put it|
|00000d60| 20 69 6e 74 6f 20 46 49 | 4c 45 50 41 54 48 0d 6f | into FI|LEPATH.o|
|00000d70| 70 65 6e 20 66 69 6c 65 | 20 46 49 4c 45 50 41 54 |pen file| FILEPAT|
|00000d80| 48 0d 70 75 74 20 62 61 | 63 6b 67 72 6f 75 6e 64 |H.put ba|ckground|
|00000d90| 20 66 69 65 6c 64 20 22 | 63 69 74 61 74 69 6f 6e | field "|citation|
|00000da0| 22 20 26 20 72 65 74 75 | 72 6e 20 26 20 72 65 74 |" & retu|rn & ret|
|00000db0| 75 72 6e 20 69 6e 74 6f | 20 63 6f 6e 74 65 6e 74 |urn into| content|
|00000dc0| 73 0d 70 75 74 20 62 61 | 63 6b 67 72 6f 75 6e 64 |s.put ba|ckground|
|00000dd0| 20 66 69 65 6c 64 20 22 | 61 62 73 74 72 61 63 74 | field "|abstract|
|00000de0| 22 20 26 20 72 65 74 75 | 72 6e 20 26 20 72 65 74 |" & retu|rn & ret|
|00000df0| 75 72 6e 20 61 66 74 65 | 72 20 63 6f 6e 74 65 6e |urn afte|r conten|
|00000e00| 74 73 0d 70 75 74 20 62 | 61 63 6b 67 72 6f 75 6e |ts.put b|ackgroun|
|00000e10| 64 20 66 69 65 6c 64 20 | 22 63 6f 6d 6d 65 6e 74 |d field |"comment|
|00000e20| 73 22 20 61 66 74 65 72 | 20 63 6f 6e 74 65 6e 74 |s" after| content|
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|000040f0| 01 00 00 a0 01 b1 00 c3 | 01 d7 20 00 00 00 09 ae |........|.. .....|
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|000046d0| ff e0 e3 2b 0f ff aa 84 | 00 00 00 00 00 00 00 00 |...+....|........|
|000046e0| 00 00 03 40 43 41 52 44 | 00 00 74 4f 00 00 7e 79 |...@CARD|..tO..~y|
|000046f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00004700| 00 00 0a 96 00 00 00 01 | 00 00 00 00 00 02 00 00 |........|........|
|00004710| 03 02 00 06 41 6c 74 6d | 61 6e 2c 20 53 2e 20 20 |....Altm|an, S.  |
|00004720| 31 39 38 39 20 20 52 69 | 62 6f 6e 75 63 6c 65 61 |1989  Ri|bonuclea|
|00004730| 73 65 20 50 3a 20 61 6e | 20 65 6e 7a 79 6d 65 20 |se P: an| enzyme |
|00004740| 77 69 74 68 20 61 20 63 | 61 74 61 6c 79 74 69 63 |with a c|atalytic|
|00004750| 20 52 4e 41 20 73 75 62 | 75 6e 69 74 2e 20 20 41 | RNA sub|unit.  A|
|00004760| 64 76 61 6e 63 65 73 20 | 69 6e 20 45 6e 7a 79 6d |dvances |in Enzym|
|00004770| 6f 6c 2e 20 36 32 3a 31 | 2d 33 36 00 00 19 41 20 |ol. 62:1|-36...A |
|00004780| 72 65 76 65 69 77 2e 0d | 0d 43 6f 6e 74 65 6e 74 |reveiw..|.Content|
|00004790| 73 3a 0d 52 69 62 6f 6e | 75 63 6c 65 61 73 65 20 |s:.Ribon|uclease |
|000047a0| 50 0d 20 20 20 20 20 49 | 6e 74 72 6f 64 75 63 74 |P.     I|ntroduct|
|000047b0| 69 6f 6e 3a 20 43 61 74 | 61 6c 79 74 69 63 20 52 |ion: Cat|alytic R|
|000047c0| 4e 41 73 0d 20 20 20 20 | 20 41 6e 20 61 63 63 6f |NAs.    | An acco|
|000047d0| 75 6e 74 20 6f 66 20 65 | 61 72 6c 79 20 73 74 75 |unt of e|arly stu|
|000047e0| 64 69 65 73 20 6f 66 20 | 72 69 62 6f 6e 75 63 6c |dies of |ribonucl|
|000047f0| 65 61 73 65 20 50 0d 20 | 20 20 20 20 50 68 79 73 |ease P. |    Phys|
|00004800| 69 63 61 6c 20 70 72 6f | 70 65 72 74 69 65 73 20 |ical pro|perties |
|00004810| 6f 66 20 52 4e 61 73 65 | 20 50 20 66 72 6f 6d 20 |of RNase| P from |
|00004820| 45 2e 63 6f 6c 69 0d 20 | 20 20 20 20 43 61 74 61 |E.coli. |    Cata|
|00004830| 6c 79 74 69 63 20 70 72 | 6f 70 65 72 74 69 65 73 |lytic pr|operties|
|00004840| 20 6f 66 20 52 4e 61 73 | 65 20 50 20 66 72 6f 6d | of RNas|e P from|
|00004850| 20 45 2e 63 6f 6c 69 0d | 52 65 63 65 6e 74 20 70 | E.coli.|Recent p|
|00004860| 72 6f 67 72 65 73 73 0d | 20 20 20 20 20 53 74 72 |rogress.|     Str|
|00004870| 75 63 74 75 72 65 2d 66 | 75 6e 63 74 69 6f 6e 20 |ucture-f|unction |
|00004880| 72 65 6c 61 74 69 6f 6e | 73 68 69 70 73 0d 20 20 |relation|ships.  |
|00004890| 20 20 20 53 75 62 73 74 | 72 61 74 65 20 72 65 63 |   Subst|rate rec|
|000048a0| 6f 67 6e 69 74 69 6f 6e | 0d 20 20 20 20 20 4d 65 |ognition|.     Me|
|000048b0| 63 68 61 6e 69 73 6d 20 | 6f 66 20 74 68 65 20 72 |chanism |of the r|
|000048c0| 65 61 63 74 69 6f 6e 0d | 20 20 20 20 20 46 75 6e |eaction.|     Fun|
|000048d0| 63 74 69 6f 6e 20 6f 66 | 20 52 4e 61 73 65 20 50 |ction of| RNase P|
|000048e0| 20 69 6e 20 76 69 74 72 | 6f 0d 20 20 20 20 20 43 | in vitr|o.     C|
|000048f0| 61 74 61 6c 79 74 69 63 | 20 52 4e 41 73 20 66 72 |atalytic| RNAs fr|
|00004900| 6f 6d 20 6f 74 68 65 72 | 20 70 72 65 70 61 72 61 |om other| prepara|
|00004910| 74 69 6f 6e 73 20 6f 66 | 20 52 4e 61 73 65 20 50 |tions of| RNase P|
|00004920| 0d 43 61 74 61 6c 79 74 | 69 63 20 52 4e 41 73 20 |.Catalyt|ic RNAs |
|00004930| 6e 6f 74 20 61 73 73 6f | 63 69 61 74 65 64 20 77 |not asso|ciated w|
|00004940| 69 74 68 20 52 4e 61 73 | 65 20 50 0d 20 20 20 20 |ith RNas|e P.    |
|00004950| 20 45 78 61 6d 70 6c 65 | 73 0d 20 20 20 20 20 20 | Example|s.      |
|00004960| 20 20 20 20 49 6e 74 72 | 6f 6e 73 0d 20 20 20 20 |    Intr|ons.    |
|00004970| 20 20 20 20 20 20 50 6c | 61 6e 74 20 76 69 72 75 |      Pl|ant viru|
|00004980| 73 6f 69 64 2c 20 73 61 | 74 65 6c 6c 69 74 65 20 |soid, sa|tellite |
|00004990| 61 6e 64 20 76 69 72 6f | 69 64 20 52 4e 41 73 0d |and viro|id RNAs.|
|000049a0| 20 20 20 20 20 20 20 20 | 20 20 45 78 6f 74 69 63 |        |  Exotic|
|000049b0| 20 72 65 61 63 74 69 6f | 6e 73 0d 20 20 20 20 20 | reactio|ns.     |
|000049c0| 43 6f 6d 70 61 72 69 73 | 6f 6e 73 0d 20 20 20 20 |Comparis|ons.    |
|000049d0| 20 52 4e 41 20 63 61 74 | 61 6c 79 73 69 73 20 61 | RNA cat|alysis a|
|000049e0| 6e 64 20 65 76 6f 6c 75 | 74 69 6f 6e 0d 43 6f 6e |nd evolu|tion.Con|
|000049f0| 63 6c 75 73 69 6f 6e 0d | 41 63 6b 6e 6f 77 6c 65 |clusion.|Acknowle|
|00004a00| 64 67 6d 65 6e 74 73 0d | 52 65 66 65 72 65 6e 63 |dgments.|Referenc|
|00004a10| 65 73 0d 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |es......|........|
|00004a20| 00 00 00 40 42 4d 41 50 | 00 00 7e 79 00 00 00 00 |...@BMAP|..~y....|
|00004a30| 00 01 00 00 00 00 00 00 | 01 56 02 00 00 38 00 1a |........|.V...8..|
|00004a40| 00 3b 00 da 00 00 00 00 | 00 00 00 00 00 00 00 00 |.;......|........|
|00004a50| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 13 94 |........|........|
|00004a60| 00 00 02 e0 43 41 52 44 | 00 00 2e db 00 00 00 00 |....CARD|........|
|00004a70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00004a80| 00 00 0a 96 00 00 00 01 | 00 00 00 00 00 03 00 00 |........|........|
|00004a90| 02 92 00 06 41 6c 74 6d | 61 6e 2c 20 53 2c 20 42 |....Altm|an, S, B|
|00004aa0| 61 65 72 2c 20 4d 2c 20 | 47 75 65 72 72 69 65 72 |aer, M, |Guerrier|
|00004ab0| 2d 54 61 6b 61 64 61 2c | 20 43 20 61 6e 64 20 56 |-Takada,| C and V|
|00004ac0| 69 6f 71 75 65 2c 20 41 | 2e 20 20 31 39 38 36 20 |ioque, A|.  1986 |
|00004ad0| 20 45 6e 7a 79 6d 61 74 | 69 63 20 63 6c 65 61 76 | Enzymat|ic cleav|
|00004ae0| 61 67 65 20 6f 66 20 52 | 4e 41 20 62 79 20 52 4e |age of R|NA by RN|
|00004af0| 41 2e 20 20 54 72 65 6e | 64 73 20 42 69 6f 63 68 |A.  Tren|ds Bioch|
|00004b00| 65 6d 2e 20 53 63 69 2e | 20 31 31 3a 35 31 35 2d |em. Sci.| 11:515-|
|00004b10| 35 31 38 00 00 18 54 68 | 65 20 63 61 74 61 6c 79 |518...Th|e cataly|
|00004b20| 74 69 63 20 61 63 74 69 | 76 69 74 79 20 6f 66 20 |tic acti|vity of |
|00004b30| 45 2e 20 63 6f 6c 69 20 | 52 4e 61 73 65 20 50 2c |E. coli |RNase P,|
|00004b40| 20 61 6e 20 65 6e 7a 79 | 6d 65 20 65 73 73 65 6e | an enzy|me essen|
|00004b50| 74 69 61 6c 20 66 6f 72 | 20 74 52 4e 41 20 62 69 |tial for| tRNA bi|
|00004b60| 6f 73 79 6e 74 68 65 73 | 69 73 20 69 6e 20 76 69 |osynthes|is in vi|
|00004b70| 76 6f 2c 20 72 65 73 69 | 64 65 73 20 69 6e 20 74 |vo, resi|des in t|
|00004b80| 68 65 20 52 4e 41 20 73 | 75 62 75 6e 69 74 20 6f |he RNA s|ubunit o|
|00004b90| 66 20 74 68 65 20 65 6e | 7a 79 6d 65 2e 20 20 54 |f the en|zyme.  T|
|00004ba0| 68 69 73 20 52 4e 41 2c | 20 77 68 69 63 68 20 68 |his RNA,| which h|
|00004bb0| 61 73 20 61 6c 6c 20 74 | 68 65 20 70 72 6f 70 65 |as all t|he prope|
|00004bc0| 72 74 69 65 73 20 6f 66 | 20 61 20 63 6c 61 73 73 |rties of| a class|
|00004bd0| 69 63 61 6c 20 65 6e 7a | 79 6d 65 2c 20 63 61 6e |ical enz|yme, can|
|00004be0| 20 63 6c 65 61 76 65 20 | 70 72 65 63 75 72 73 6f | cleave |precurso|
|00004bf0| 72 20 74 52 4e 41 73 20 | 69 6e 20 76 69 74 72 6f |r tRNAs |in vitro|
|00004c00| 20 69 6e 20 74 68 65 20 | 74 6f 74 61 6c 20 61 62 | in the |total ab|
|00004c10| 73 65 6e 63 65 20 6f 66 | 20 70 72 6f 74 65 69 6e |sence of| protein|
|00004c20| 73 2e 00 00 19 41 20 72 | 65 76 69 65 77 20 2d 20 |s....A r|eview - |
|00004c30| 63 6f 6e 74 61 69 6e 73 | 3a 0d 0d 31 2e 20 43 61 |contains|:..1. Ca|
|00004c40| 74 61 6c 79 74 69 63 20 | 52 4e 41 0d 32 2e 20 50 |talytic |RNA.2. P|
|00004c50| 72 6f 70 65 72 74 69 65 | 73 20 6f 66 20 74 68 65 |ropertie|s of the|
|00004c60| 20 72 65 61 63 74 69 6f | 6e 20 63 61 74 61 6c 79 | reactio|n cataly|
|00004c70| 7a 65 64 20 62 79 20 4d | 31 20 52 4e 41 2e 0d 33 |zed by M|1 RNA..3|
|00004c80| 2e 20 54 68 65 20 73 74 | 72 75 63 74 75 72 65 20 |. The st|ructure |
|00004c90| 6f 66 20 4d 31 20 52 4e | 41 2e 0d 34 2e 20 4f 6e |of M1 RN|A..4. On|
|00004ca0| 65 20 74 68 65 6f 72 79 | 20 6f 66 20 73 75 62 73 |e theory| of subs|
|00004cb0| 74 72 61 74 65 20 72 65 | 63 6f 67 6e 69 74 69 6f |trate re|cognitio|
|00004cc0| 6e 2e 0d 35 2e 20 46 75 | 6e 63 74 69 6f 6e 20 6f |n..5. Fu|nction o|
|00004cd0| 66 20 70 61 72 74 69 61 | 6c 20 6d 6f 6c 65 63 75 |f partia|l molecu|
|00004ce0| 6c 65 73 20 61 6e 64 20 | 6d 75 74 61 6e 74 73 20 |les and |mutants |
|00004cf0| 6f 66 20 4d 31 20 52 4e | 41 2e 0d 36 2e 20 54 68 |of M1 RN|A..6. Th|
|00004d00| 65 20 72 6f 6c 65 20 6f | 66 20 43 35 20 70 72 6f |e role o|f C5 pro|
|00004d10| 74 65 69 6e 2e 0d 37 2e | 20 43 6f 6e 63 6c 75 73 |tein..7.| Conclus|
|00004d20| 69 6f 6e 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |ion.....|........|
|00004d30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00004d40| 00 00 03 c0 43 41 52 44 | 00 00 1f da 00 00 00 00 |....CARD|........|
|00004d50| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00004d60| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 02 00 00 |........|........|
|00004d70| 03 74 00 06 41 6c 74 6d | 61 6e 2c 20 53 2e 20 61 |.t..Altm|an, S. a|
|00004d80| 6e 64 20 47 75 65 72 72 | 69 65 72 2d 54 61 6b 61 |nd Guerr|ier-Taka|
|00004d90| 64 61 2c 20 43 2e 20 20 | 31 39 38 36 20 20 4d 31 |da, C.  |1986  M1|
|00004da0| 20 52 4e 41 2c 20 74 68 | 65 20 52 4e 41 20 73 75 | RNA, th|e RNA su|
|00004db0| 62 75 6e 69 74 20 6f 66 | 20 45 73 63 68 65 72 69 |bunit of| Escheri|
|00004dc0| 63 68 69 61 20 63 6f 6c | 69 20 72 69 62 6f 6e 75 |chia col|i ribonu|
|00004dd0| 63 6c 65 61 73 65 20 50 | 2c 20 63 61 6e 20 75 6e |clease P|, can un|
|00004de0| 64 65 72 67 6f 20 61 20 | 70 48 2d 73 65 6e 73 69 |dergo a |pH-sensi|
|00004df0| 74 69 76 65 20 63 6f 6e | 66 6f 72 6d 61 74 69 6f |tive con|formatio|
|00004e00| 6e 61 6c 20 63 68 61 6e | 67 65 2e 20 20 42 69 6f |nal chan|ge.  Bio|
|00004e10| 63 68 65 6d 69 73 74 72 | 79 20 32 35 3a 31 32 30 |chemistr|y 25:120|
|00004e20| 35 2d 31 32 30 38 00 00 | 18 41 66 74 65 72 20 70 |5-1208..|.After p|
|00004e30| 75 72 69 66 69 63 61 74 | 69 6f 6e 20 66 72 6f 6d |urificat|ion from|
|00004e40| 20 65 78 74 72 61 63 74 | 73 20 6f 66 20 77 68 6f | extract|s of who|
|00004e50| 6c 65 20 63 65 6c 6c 73 | 2c 20 4d 31 20 52 4e 41 |le cells|, M1 RNA|
|00004e60| 2c 20 74 68 65 20 63 61 | 74 61 6c 79 74 69 63 20 |, the ca|talytic |
|00004e70| 73 75 62 75 6e 69 74 20 | 6f 66 20 72 69 62 6f 6e |subunit |of ribon|
|00004e80| 75 63 6c 65 61 73 65 20 | 50 20 66 72 6f 6d 20 45 |uclease |P from E|
|00004e90| 73 63 68 65 72 69 63 68 | 69 61 20 63 6f 6c 69 2c |scherich|ia coli,|
|00004ea0| 20 61 70 70 61 72 65 6e | 74 6c 79 20 6d 75 73 74 | apparen|tly must|
|00004eb0| 20 75 6e 64 65 72 67 6f | 20 61 20 63 68 61 6e 67 | undergo| a chang|
|00004ec0| 65 20 69 6e 20 63 6f 6e | 66 6f 72 6d 61 74 69 6f |e in con|formatio|
|00004ed0| 6e 20 62 65 66 6f 72 65 | 20 69 74 20 63 61 6e 20 |n before| it can |
|00004ee0| 66 75 6e 63 74 69 6f 6e | 20 63 61 74 61 6c 79 74 |function| catalyt|
|00004ef0| 69 63 61 6c 6c 79 2e 20 | 20 54 68 65 20 72 61 74 |ically. | The rat|
|00004f00| 65 20 6f 66 20 74 68 69 | 73 20 63 6f 6e 66 6f 72 |e of thi|s confor|
|00004f10| 6d 61 74 69 6f 6e 61 6c | 20 63 68 61 6e 67 65 20 |mational| change |
|00004f20| 69 73 20 64 65 70 65 6e | 64 65 6e 74 20 75 70 6f |is depen|dent upo|
|00004f30| 6e 20 74 68 65 20 64 75 | 72 61 74 69 6f 6e 20 6f |n the du|ration o|
|00004f40| 66 20 69 6e 63 75 62 61 | 74 69 6f 6e 20 61 74 20 |f incuba|tion at |
|00004f50| 76 61 72 69 6f 75 73 20 | 74 65 6d 70 65 72 61 74 |various |temperat|
|00004f60| 75 72 65 73 20 61 6e 64 | 20 70 48 2e 20 20 44 65 |ures and| pH.  De|
|00004f70| 6c 74 61 45 2b 2b 20 6f | 66 20 74 68 65 20 74 72 |ltaE++ o|f the tr|
|00004f80| 61 6e 73 69 74 69 6f 6e | 20 61 74 20 70 48 37 2e |ansition| at pH7.|
|00004f90| 35 20 69 73 20 61 70 70 | 72 6f 78 69 6d 61 74 65 |5 is app|roximate|
|00004fa0| 6c 79 20 33 36 4b 63 61 | 6c 2f 6d 6f 6c 2e 20 20 |ly 36Kca|l/mol.  |
|00004fb0| 54 68 65 20 63 68 61 6e | 67 65 20 69 6e 20 63 6f |The chan|ge in co|
|00004fc0| 6e 66 6f 72 6d 61 74 69 | 6f 6e 20 69 73 20 6e 6f |nformati|on is no|
|00004fd0| 74 20 73 65 6e 73 69 74 | 69 76 65 20 74 6f 20 4d |t sensit|ive to M|
|00004fe0| 67 2b 2b 20 63 6f 6e 63 | 65 6e 74 72 61 74 69 6f |g++ conc|entratio|
|00004ff0| 6e 20 62 65 74 77 65 65 | 6e 20 31 30 20 61 6e 64 |n betwee|n 10 and|
|00005000| 20 31 30 30 6d 4d 2e 20 | 20 41 20 64 65 63 72 65 | 100mM. | A decre|
|00005010| 61 73 65 20 69 6e 20 41 | 32 36 30 20 6f 66 20 4d |ase in A|260 of M|
|00005020| 31 20 52 4e 41 20 69 6e | 20 73 6f 6c 75 74 69 6f |1 RNA in| solutio|
|00005030| 6e 20 68 61 73 20 62 65 | 65 6e 20 6f 62 73 65 72 |n has be|en obser|
|00005040| 76 65 64 20 64 75 72 69 | 6e 67 20 74 68 65 20 69 |ved duri|ng the i|
|00005050| 6e 63 75 62 61 74 69 6f | 6e 20 70 65 72 69 6f 64 |ncubatio|n period|
|00005060| 20 74 68 61 74 20 70 6f | 74 65 6e 74 69 61 74 65 | that po|tentiate|
|00005070| 73 20 74 68 65 20 63 6f | 6e 66 6f 72 6d 61 74 69 |s the co|nformati|
|00005080| 6f 6e 61 6c 20 63 68 61 | 6e 67 65 20 61 74 20 33 |onal cha|nge at 3|
|00005090| 30 6f 43 2c 20 62 75 74 | 20 6e 6f 20 64 69 72 65 |0oC, but| no dire|
|000050a0| 63 74 20 63 6f 72 72 65 | 6c 61 74 69 6f 6e 20 63 |ct corre|lation c|
|000050b0| 61 6e 20 79 65 74 20 62 | 65 20 6d 61 64 65 20 74 |an yet b|e made t|
|000050c0| 6f 20 73 70 65 63 69 66 | 69 63 20 73 74 72 75 63 |o specif|ic struc|
|000050d0| 74 75 72 61 6c 20 72 65 | 61 72 72 61 6e 67 65 6d |tural re|arrangem|
|000050e0| 65 6e 74 73 2e 00 00 00 | 00 00 00 00 00 00 00 00 |ents....|........|
|000050f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00005100| 00 00 01 e0 43 41 52 44 | 00 00 75 78 00 00 00 00 |....CARD|..ux....|
|00005110| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00005120| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 02 00 00 |........|........|
|00005130| 01 9d 00 06 41 6c 74 6d | 61 6e 2c 20 53 2e 20 61 |....Altm|an, S. a|
|00005140| 6e 64 20 53 6d 69 74 68 | 2c 20 4a 2e 20 44 2e 20 |nd Smith|, J. D. |
|00005150| 20 31 39 37 31 20 20 54 | 79 72 6f 73 69 6e 65 20 | 1971  T|yrosine |
|00005160| 74 52 4e 41 20 70 72 65 | 63 75 72 73 6f 72 20 6d |tRNA pre|cursor m|
|00005170| 6f 6c 65 63 75 6c 65 20 | 70 6f 6c 79 6e 75 63 6c |olecule |polynucl|
|00005180| 65 6f 74 69 64 65 20 73 | 65 71 75 65 6e 63 65 2e |eotide s|equence.|
|00005190| 20 20 4e 61 74 75 72 65 | 20 4e 65 77 20 42 69 6f |  Nature| New Bio|
|000051a0| 6c 2e 20 32 33 33 3a 33 | 35 2d 33 39 00 00 18 54 |l. 233:3|5-39...T|
|000051b0| 68 65 20 74 72 61 6e 73 | 63 72 69 62 65 64 20 70 |he trans|cribed p|
|000051c0| 72 65 63 75 72 73 6f 72 | 20 6f 66 20 61 6e 20 45 |recursor| of an E|
|000051d0| 2e 63 6f 6c 69 20 74 79 | 72 6f 73 69 6e 65 20 74 |.coli ty|rosine t|
|000051e0| 52 4e 41 20 63 6f 6e 74 | 61 69 6e 73 20 6e 75 63 |RNA cont|ains nuc|
|000051f0| 6c 65 6f 74 69 64 65 20 | 73 65 71 75 65 6e 63 65 |leotide |sequence|
|00005200| 73 20 6e 6f 74 20 66 6f | 75 6e 64 20 69 6e 20 74 |s not fo|und in t|
|00005210| 68 65 20 6d 61 74 75 72 | 65 20 74 52 4e 41 73 2e |he matur|e tRNAs.|
|00005220| 20 20 45 76 69 64 65 6e | 63 65 20 73 75 67 67 65 |  Eviden|ce sugge|
|00005230| 73 74 73 20 74 68 61 74 | 20 74 68 65 73 65 20 72 |sts that| these r|
|00005240| 65 67 69 6f 6e 73 2c 20 | 77 68 69 63 68 20 63 61 |egions, |which ca|
|00005250| 6e 20 62 65 20 74 72 69 | 6d 6d 65 64 20 69 6e 20 |n be tri|mmed in |
|00005260| 76 69 74 72 6f 20 62 79 | 20 61 20 6e 75 63 6c 65 |vitro by| a nucle|
|00005270| 61 73 65 20 61 63 74 69 | 76 69 74 79 20 69 6e 20 |ase acti|vity in |
|00005280| 63 72 75 64 65 20 63 65 | 6c 6c 20 65 78 74 72 61 |crude ce|ll extra|
|00005290| 63 74 73 2c 20 61 72 65 | 20 69 6e 76 6f 6c 76 65 |cts, are| involve|
|000052a0| 64 20 69 6e 20 74 68 65 | 20 63 6f 6e 74 72 6f 6c |d in the| control|
|000052b0| 20 6f 66 20 63 65 6c 6c | 20 74 79 72 6f 73 69 6e | of cell| tyrosin|
|000052c0| 65 20 74 52 4e 41 20 6c | 65 76 65 6c 73 2e 00 00 |e tRNA l|evels...|
|000052d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000052e0| 00 00 03 00 43 41 52 44 | 00 00 76 e1 00 00 00 00 |....CARD|..v.....|
|000052f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00005300| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 03 00 00 |........|........|
|00005310| 02 be 00 06 41 70 69 72 | 69 6f 6e 2c 20 44 2e 20 |....Apir|ion, D. |
|00005320| 20 31 39 38 30 20 20 47 | 65 6e 65 74 69 63 20 6d | 1980  G|enetic m|
|00005330| 61 70 70 69 6e 67 20 61 | 6e 64 20 73 6f 6d 65 20 |apping a|nd some |
|00005340| 63 68 61 72 61 63 74 65 | 72 69 7a 61 74 69 6f 6e |characte|rization|
|00005350| 20 6f 66 20 74 68 65 20 | 72 6e 70 41 34 39 20 6d | of the |rnpA49 m|
|00005360| 75 74 61 74 69 6f 6e 20 | 74 68 61 74 20 61 66 66 |utation |that aff|
|00005370| 65 63 74 73 20 74 68 65 | 20 52 4e 41 2d 70 72 6f |ects the| RNA-pro|
|00005380| 63 65 73 73 69 6e 67 20 | 65 6e 7a 79 6d 65 20 72 |cessing |enzyme r|
|00005390| 69 62 6f 6e 75 63 6c 65 | 61 73 65 20 50 2e 20 20 |ibonucle|ase P.  |
|000053a0| 47 65 6e 65 74 69 63 73 | 20 39 34 3a 32 39 31 2d |Genetics| 94:291-|
|000053b0| 32 39 39 00 00 19 69 6e | 66 6f 20 6f 6e 20 74 68 |299...in|fo on th|
|000053c0| 65 20 46 53 31 30 31 20 | 6d 75 74 61 74 69 6f 6e |e FS101 |mutation|
|000053d0| 00 00 18 41 20 6d 75 74 | 61 6e 74 20 64 65 66 65 |...A mut|ant defe|
|000053e0| 63 74 69 76 65 20 69 6e | 20 74 68 65 20 65 6e 7a |ctive in| the enz|
|000053f0| 79 6d 65 20 52 4e 61 73 | 65 20 50 20 77 61 73 20 |yme RNas|e P was |
|00005400| 69 73 6f 6c 61 74 65 64 | 20 62 79 20 50 2e 20 53 |isolated| by P. S|
|00005410| 63 68 65 64 6c 20 61 6e | 64 20 50 2e 20 50 72 69 |chedl an|d P. Pri|
|00005420| 6d 61 6b 6f 66 66 20 28 | 31 39 37 33 29 2e 20 20 |makoff (|1973).  |
|00005430| 54 68 65 20 6d 75 74 61 | 74 69 6f 6e 20 72 6e 70 |The muta|tion rnp|
|00005440| 41 34 39 20 66 6f 75 6e | 64 20 69 6e 20 74 68 69 |A49 foun|d in thi|
|00005450| 73 20 73 74 72 61 69 6e | 2c 20 77 68 69 63 68 20 |s strain|, which |
|00005460| 63 6f 6e 66 65 72 73 20 | 74 65 6d 70 65 72 61 74 |confers |temperat|
|00005470| 75 72 65 20 73 65 6e 73 | 69 74 69 76 69 74 79 20 |ure sens|itivity |
|00005480| 6f 6e 20 63 61 72 72 69 | 65 72 20 73 74 72 61 69 |on carri|er strai|
|00005490| 6e 73 2c 20 77 61 73 20 | 6d 61 70 70 65 64 20 62 |ns, was |mapped b|
|000054a0| 79 20 63 6f 6e 6a 75 67 | 61 74 69 6f 6e 20 61 6e |y conjug|ation an|
|000054b0| 64 20 74 72 61 6e 73 64 | 75 63 74 69 6f 6e 20 65 |d transd|uction e|
|000054c0| 78 70 65 72 69 6d 65 6e | 74 73 20 61 6e 64 20 6c |xperimen|ts and l|
|000054d0| 6f 63 61 74 65 64 20 61 | 72 6f 75 6e 64 20 6d 69 |ocated a|round mi|
|000054e0| 6e 75 74 65 20 38 32 20 | 6f 66 20 74 68 65 20 45 |nute 82 |of the E|
|000054f0| 2e 63 6f 6c 69 20 6d 61 | 70 2c 20 77 69 74 68 20 |.coli ma|p, with |
|00005500| 74 68 65 20 73 75 67 67 | 65 73 74 65 64 20 6f 72 |the sugg|ested or|
|00005510| 64 65 72 20 72 6e 70 41 | 20 62 67 6c 42 20 70 68 |der rnpA| bglB ph|
|00005520| 6f 53 20 72 62 73 50 20 | 69 6c 76 2e 20 20 41 73 |oS rbsP |ilv.  As|
|00005530| 20 65 78 70 65 63 74 65 | 64 2c 20 74 68 65 20 72 | expecte|d, the r|
|00005540| 6e 70 41 34 39 20 6d 75 | 74 61 74 69 6f 6e 20 69 |npA49 mu|tation i|
|00005550| 73 20 72 65 63 65 73 73 | 69 76 65 2e 20 20 45 76 |s recess|ive.  Ev|
|00005560| 65 6e 20 74 68 6f 75 67 | 68 20 74 68 69 73 20 6d |en thoug|h this m|
|00005570| 75 74 61 74 69 6f 6e 20 | 69 73 20 63 6f 6e 64 69 |utation |is condi|
|00005580| 74 69 6f 6e 61 6c 2c 20 | 69 74 20 69 73 20 6d 61 |tional, |it is ma|
|00005590| 6e 69 66 65 73 74 65 64 | 20 61 74 20 74 65 6d 70 |nifested| at temp|
|000055a0| 65 72 61 74 75 72 65 73 | 20 61 74 20 77 68 69 63 |eratures| at whic|
|000055b0| 68 20 74 68 65 20 63 61 | 72 72 69 65 72 20 73 74 |h the ca|rrier st|
|000055c0| 72 61 69 6e 73 20 63 61 | 6e 20 67 72 6f 77 2e 00 |rains ca|n grow..|
|000055d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000055e0| 00 00 03 20 43 41 52 44 | 00 00 77 fd 00 00 00 00 |... CARD|..w.....|
|000055f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00005600| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 03 00 00 |........|........|
|00005610| 02 d7 00 06 41 70 69 72 | 69 6f 6e 2c 20 44 20 61 |....Apir|ion, D a|
|00005620| 6e 64 20 57 61 74 73 6f | 6e 2c 20 4e 2e 20 20 31 |nd Watso|n, N.  1|
|00005630| 39 38 30 20 20 41 20 73 | 65 63 6f 6e 64 20 67 65 |980  A s|econd ge|
|00005640| 6e 65 20 77 68 69 63 68 | 20 61 66 66 65 63 74 73 |ne which| affects|
|00005650| 20 74 68 65 20 52 4e 41 | 20 70 72 6f 63 65 73 73 | the RNA| process|
|00005660| 69 6e 67 20 65 6e 7a 79 | 6d 65 20 72 69 62 6f 6e |ing enzy|me ribon|
|00005670| 75 63 6c 65 61 73 65 20 | 50 20 6f 66 20 45 73 63 |uclease |P of Esc|
|00005680| 68 65 72 69 63 68 69 61 | 20 63 6f 6c 69 2e 20 20 |herichia| coli.  |
|00005690| 46 45 42 53 20 4c 65 74 | 74 2e 20 31 31 30 3a 31 |FEBS Let|t. 110:1|
|000056a0| 36 31 2d 31 36 33 00 00 | 18 54 68 69 73 20 69 73 |61-163..|.This is|
|000056b0| 20 74 68 65 20 49 6e 74 | 72 6f 64 75 63 74 69 6f | the Int|roductio|
|000056c0| 6e 3a 0d 0d 54 68 65 20 | 45 73 63 68 65 72 69 63 |n:..The |Escheric|
|000056d0| 68 69 61 20 63 6f 6c 69 | 20 52 4e 41 20 70 72 6f |hia coli| RNA pro|
|000056e0| 63 65 73 73 69 6e 67 20 | 65 6e 7a 79 6d 65 20 52 |cessing |enzyme R|
|000056f0| 4e 61 73 65 20 50 20 77 | 61 73 20 73 68 6f 77 6e |Nase P w|as shown|
|00005700| 20 74 6f 20 70 61 72 74 | 69 63 69 70 61 74 65 20 | to part|icipate |
|00005710| 69 6e 20 70 72 6f 63 65 | 73 73 69 6e 67 20 6f 66 |in proce|ssing of|
|00005720| 20 52 4e 41 20 5b 31 5d | 2e 20 20 61 6e 64 20 72 | RNA [1]|.  and r|
|00005730| 52 4e 41 20 5b 32 5d 2e | 20 20 4d 75 74 61 74 69 |RNA [2].|  Mutati|
|00005740| 6f 6e 73 20 77 68 69 63 | 68 20 61 66 66 65 63 74 |ons whic|h affect|
|00005750| 20 74 68 69 73 20 65 6e | 7a 79 6d 65 20 77 65 72 | this en|zyme wer|
|00005760| 65 20 69 73 6f 6c 61 74 | 65 64 20 69 6e 20 5b 33 |e isolat|ed in [3|
|00005770| 2c 34 5d 2e 20 20 52 65 | 63 65 6e 74 6c 79 20 61 |,4].  Re|cently a|
|00005780| 20 6d 75 74 61 74 69 6f | 6e 20 61 66 66 65 63 74 | mutatio|n affect|
|00005790| 69 6e 67 20 52 4e 61 73 | 65 20 50 2c 20 69 73 6f |ing RNas|e P, iso|
|000057a0| 6c 61 74 65 64 20 5b 33 | 5d 20 77 61 73 20 6d 61 |lated [3|] was ma|
|000057b0| 70 70 65 64 20 6e 65 61 | 72 20 6d 69 6e 20 38 32 |pped nea|r min 82|
|000057c0| 20 6f 66 20 74 68 65 20 | 45 2e 63 6f 6c 69 20 63 | of the |E.coli c|
|000057d0| 68 72 6f 6d 6f 73 6f 6d | 65 20 5b 35 5d 2e 20 20 |hromosom|e [5].  |
|000057e0| 54 68 69 73 20 6d 75 74 | 61 74 69 6f 6e 20 77 61 |This mut|ation wa|
|000057f0| 73 20 64 65 73 69 67 6e | 61 74 65 64 20 72 6e 70 |s design|ated rnp|
|00005800| 41 34 39 2e 20 20 28 66 | 6f 72 20 6e 6f 6d 65 6e |A49.  (f|or nomen|
|00005810| 63 6c 61 74 75 72 65 20 | 6f 66 20 6d 75 74 61 74 |clature |of mutat|
|00005820| 69 6f 6e 73 20 61 66 66 | 65 63 74 69 6e 67 20 52 |ions aff|ecting R|
|00005830| 4e 61 73 65 73 20 73 65 | 65 20 5b 36 5d 2e 29 20 |Nases se|e [6].) |
|00005840| 20 48 65 72 65 20 77 65 | 20 73 68 61 6c 6c 20 64 | Here we| shall d|
|00005850| 65 73 63 72 69 62 65 20 | 74 68 65 20 69 73 6f 6c |escribe |the isol|
|00005860| 61 74 69 6f 6e 20 6f 66 | 20 61 20 73 65 63 6f 6e |ation of| a secon|
|00005870| 64 20 6d 75 74 61 74 69 | 6f 6e 20 77 68 69 63 68 |d mutati|on which|
|00005880| 20 61 66 66 65 63 74 73 | 20 52 4e 61 73 65 20 50 | affects| RNase P|
|00005890| 20 61 6e 64 20 73 68 6f | 77 20 74 68 61 74 20 69 | and sho|w that i|
|000058a0| 74 20 6d 61 70 73 20 6e | 65 61 72 20 6d 69 6e 20 |t maps n|ear min |
|000058b0| 36 38 20 6f 66 20 74 68 | 65 20 45 2e 63 6f 6c 69 |68 of th|e E.coli|
|000058c0| 20 63 68 72 6f 6d 6f 73 | 6f 6d 65 20 5b 37 5d 2e | chromos|ome [7].|
|000058d0| 00 00 19 54 68 65 20 52 | 4e 61 73 65 20 50 20 52 |...The R|Nase P R|
|000058e0| 4e 41 20 67 65 6e 65 2e | 00 00 00 00 00 00 00 00 |NA gene.|........|
|000058f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00005900| 00 00 04 20 43 41 52 44 | 00 00 5d da 00 00 00 00 |... CARD|..].....|
|00005910| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00005920| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 03 00 00 |........|........|
|00005930| 03 d6 00 06 41 72 72 69 | 67 6f 2c 20 41 2e 20 50 |....Arri|go, A. P|
|00005940| 2e 2c 20 53 69 6d 6f 6e | 2c 20 4d 2e 2c 20 44 61 |., Simon|, M., Da|
|00005950| 72 6c 69 78 2c 20 4a 2e | 20 4c 2e 20 61 6e 64 20 |rlix, J.| L. and |
|00005960| 53 70 61 68 72 2c 20 50 | 2e 20 46 2e 20 20 31 39 |Spahr, P|. F.  19|
|00005970| 38 37 20 20 41 20 32 30 | 53 20 70 61 72 74 69 63 |87  A 20|S partic|
|00005980| 6c 65 20 75 62 69 71 75 | 69 74 6f 75 73 20 66 72 |le ubiqu|itous fr|
|00005990| 6f 6d 20 79 65 61 73 74 | 20 74 6f 20 68 75 6d 61 |om yeast| to huma|
|000059a0| 6e 2e 20 20 4a 2e 20 4d | 6f 6c 2e 20 45 76 6f 6c |n.  J. M|ol. Evol|
|000059b0| 2e 20 32 35 3a 31 34 31 | 2d 31 35 30 00 00 18 57 |. 25:141|-150...W|
|000059c0| 65 20 68 61 76 65 20 70 | 75 72 69 66 69 65 64 20 |e have p|urified |
|000059d0| 61 6e 64 20 63 68 61 72 | 61 63 74 65 72 69 7a 65 |and char|acterize|
|000059e0| 64 20 61 20 70 61 72 74 | 69 63 6c 65 20 73 65 64 |d a part|icle sed|
|000059f0| 69 6d 65 6e 74 69 6e 67 | 20 61 74 20 32 30 53 20 |imenting| at 20S |
|00005a00| 66 72 6f 6d 20 74 68 65 | 20 70 6f 73 74 72 69 62 |from the| postrib|
|00005a10| 6f 73 6f 6d 61 6c 20 66 | 72 61 63 74 69 6f 6e 20 |osomal f|raction |
|00005a20| 6f 66 20 79 65 61 73 74 | 2c 20 77 68 65 61 74 20 |of yeast|, wheat |
|00005a30| 67 65 72 6d 2c 20 44 72 | 6f 73 6f 70 68 69 6c 61 |germ, Dr|osophila|
|00005a40| 20 6d 65 6c 61 6e 6f 67 | 61 73 74 65 72 20 74 69 | melanog|aster ti|
|00005a50| 73 73 75 65 20 63 75 6c | 74 75 72 65 20 63 65 6c |ssue cul|ture cel|
|00005a60| 6c 73 20 2c 20 63 68 69 | 63 6b 65 6e 20 65 6d 62 |ls , chi|cken emb|
|00005a70| 72 79 6f 20 66 69 62 72 | 6f 62 6c 61 73 74 73 2c |ryo fibr|oblasts,|
|00005a80| 20 72 61 62 62 69 74 20 | 72 65 74 69 63 75 6c 6f | rabbit |reticulo|
|00005a90| 63 79 74 65 20 6c 79 73 | 61 74 65 2c 20 61 6e 64 |cyte lys|ate, and|
|00005aa0| 20 48 65 4c 61 20 63 65 | 6c 6c 73 2e 20 20 4d 6f | HeLa ce|lls.  Mo|
|00005ab0| 73 74 20 6f 66 20 74 68 | 65 20 70 72 6f 74 65 69 |st of th|e protei|
|00005ac0| 6e 20 63 6f 6e 73 74 69 | 74 75 65 6e 74 73 20 6f |n consti|tuents o|
|00005ad0| 66 20 74 68 65 20 32 30 | 53 20 70 61 72 74 69 63 |f the 20|S partic|
|00005ae0| 6c 65 20 68 61 76 65 20 | 6d 6f 6c 65 63 75 6c 61 |le have |molecula|
|00005af0| 72 20 77 65 69 67 68 74 | 73 20 6f 66 20 32 30 2d |r weight|s of 20-|
|00005b00| 33 35 20 6b 64 20 61 6e | 64 20 64 69 66 66 65 72 |35 kd an|d differ|
|00005b10| 20 62 65 74 77 65 65 6e | 20 73 70 65 63 69 65 73 | between| species|
|00005b20| 3b 20 68 6f 77 65 76 65 | 72 2c 20 73 6f 6d 65 20 |; howeve|r, some |
|00005b30| 64 6f 20 68 61 76 65 20 | 73 69 6d 69 6c 61 72 20 |do have |similar |
|00005b40| 6d 6f 6c 65 63 75 6c 61 | 72 20 77 65 69 67 68 74 |molecula|r weight|
|00005b50| 73 20 61 6e 64 20 69 73 | 6f 65 6c 65 63 74 72 69 |s and is|oelectri|
|00005b60| 63 20 70 6f 69 6e 74 73 | 2c 20 73 75 67 67 65 73 |c points|, sugges|
|00005b70| 74 69 6e 67 20 74 68 61 | 74 20 74 68 65 79 20 61 |ting tha|t they a|
|00005b80| 72 65 20 72 65 6c 61 74 | 65 64 2e 20 20 53 65 76 |re relat|ed.  Sev|
|00005b90| 65 72 61 6c 20 6c 6f 77 | 20 6d 6f 6c 65 63 75 6c |eral low| molecul|
|00005ba0| 61 72 20 77 65 69 67 68 | 74 20 52 4e 41 73 2c 20 |ar weigh|t RNAs, |
|00005bb0| 64 69 73 74 69 6e 63 74 | 20 66 72 6f 6d 20 74 52 |distinct| from tR|
|00005bc0| 4e 41 73 2c 20 63 6f 2d | 70 75 72 69 66 79 20 77 |NAs, co-|purify w|
|00005bd0| 69 74 68 20 74 68 65 20 | 70 61 72 74 69 63 6c 65 |ith the |particle|
|00005be0| 20 69 73 6f 6c 61 74 65 | 64 20 66 72 6f 6d 20 61 | isolate|d from a|
|00005bf0| 6c 6c 20 74 68 65 73 65 | 20 73 70 65 63 69 65 73 |ll these| species|
|00005c00| 20 61 6e 64 20 73 68 6f | 77 20 69 6e 63 72 65 61 | and sho|w increa|
|00005c10| 73 69 6e 67 6c 79 20 6d | 6f 72 65 20 63 6f 6d 70 |singly m|ore comp|
|00005c20| 6c 65 78 20 70 61 74 74 | 65 72 6e 73 20 61 73 63 |lex patt|erns asc|
|00005c30| 65 6e 64 69 6e 67 20 74 | 68 65 20 61 72 62 69 74 |ending t|he arbit|
|00005c40| 72 61 72 79 20 6f 72 64 | 65 72 20 66 72 6f 6d 20 |rary ord|er from |
|00005c50| 79 65 61 73 74 20 74 6f | 20 68 75 6d 61 6e 20 28 |yeast to| human (|
|00005c60| 79 65 61 73 74 2c 20 70 | 6c 61 6e 74 2c 20 69 6e |yeast, p|lant, in|
|00005c70| 73 65 63 74 2c 20 62 69 | 72 64 2c 20 61 6e 64 20 |sect, bi|rd, and |
|00005c80| 6d 61 6d 6d 61 6c 73 29 | 2e 20 20 49 6e 20 44 72 |mammals)|.  In Dr|
|00005c90| 6f 73 6f 70 68 69 6c 61 | 2c 20 77 65 20 70 72 65 |osophila|, we pre|
|00005ca0| 73 65 6e 74 20 65 76 69 | 64 65 6e 63 65 20 74 68 |sent evi|dence th|
|00005cb0| 61 74 20 74 68 65 73 65 | 20 73 6d 61 6c 20 52 4e |at these| smal RN|
|00005cc0| 41 73 20 61 72 65 20 74 | 69 67 68 74 6c 79 20 61 |As are t|ightly a|
|00005cd0| 73 73 6f 63 69 61 74 65 | 64 20 77 69 74 68 20 74 |ssociate|d with t|
|00005ce0| 68 69 73 20 32 30 53 20 | 73 74 72 75 63 74 75 72 |his 20S |structur|
|00005cf0| 65 2e 00 00 19 22 66 75 | 6e 63 74 69 6f 6e 20 75 |e...."fu|nction u|
|00005d00| 6e 6b 6e 6f 77 6e 22 00 | 00 00 00 00 00 00 00 00 |nknown".|........|
|00005d10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00005d20| 00 00 05 00 43 41 52 44 | 00 00 42 6c 00 00 00 00 |....CARD|..Bl....|
|00005d30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00005d40| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 03 00 00 |........|........|
|00005d50| 04 b9 00 06 42 61 65 72 | 2c 20 4d 2e 20 61 6e 64 |....Baer|, M. and|
|00005d60| 20 41 6c 74 6d 61 6e 2c | 20 53 2e 20 20 31 39 38 | Altman,| S.  198|
|00005d70| 35 20 20 41 20 63 61 74 | 61 6c 79 74 69 63 20 52 |5  A cat|alytic R|
|00005d80| 4e 41 20 61 6e 64 20 69 | 74 73 20 67 65 6e 65 20 |NA and i|ts gene |
|00005d90| 66 72 6f 6d 20 53 61 6c | 6d 6f 6e 65 6c 6c 61 20 |from Sal|monella |
|00005da0| 74 79 70 68 69 6d 75 72 | 69 75 6d 2e 20 20 53 63 |typhimur|ium.  Sc|
|00005db0| 69 65 6e 63 65 20 32 32 | 38 3a 39 39 39 2d 31 30 |ience 22|8:999-10|
|00005dc0| 30 32 00 00 18 54 68 65 | 20 67 65 6e 65 20 66 6f |02...The| gene fo|
|00005dd0| 72 20 74 68 65 20 52 4e | 41 20 73 75 62 75 6e 69 |r the RN|A subuni|
|00005de0| 74 20 28 4d 31 20 52 4e | 41 29 20 6f 66 20 72 69 |t (M1 RN|A) of ri|
|00005df0| 62 6f 6e 75 63 6c 65 61 | 73 65 20 50 20 66 72 6f |bonuclea|se P fro|
|00005e00| 6d 20 53 61 6c 6d 6f 6e | 65 6c 6c 61 20 74 79 70 |m Salmon|ella typ|
|00005e10| 68 69 6d 75 72 69 75 6d | 20 64 69 72 65 63 74 73 |himurium| directs|
|00005e20| 20 74 68 65 20 73 79 6e | 74 68 65 73 69 73 20 6f | the syn|thesis o|
|00005e30| 66 20 61 6e 20 52 4e 41 | 20 74 68 61 74 20 63 61 |f an RNA| that ca|
|00005e40| 6e 20 63 6c 65 61 76 65 | 20 74 72 61 6e 73 66 65 |n cleave| transfe|
|00005e50| 72 20 52 4e 41 20 70 72 | 65 63 75 72 73 6f 72 20 |r RNA pr|ecursor |
|00005e60| 6d 6f 6c 65 63 75 6c 65 | 73 2e 20 20 54 68 65 20 |molecule|s.  The |
|00005e70| 6d 61 74 75 72 65 20 4d | 31 20 52 4e 41 20 63 6f |mature M|1 RNA co|
|00005e80| 64 65 64 20 66 6f 72 20 | 62 79 20 53 61 6c 6d 6f |ded for |by Salmo|
|00005e90| 6e 65 6c 6c 61 20 74 79 | 70 68 69 6d 75 72 69 75 |nella ty|phimuriu|
|00005ea0| 6d 20 69 73 20 33 37 35 | 20 6e 75 63 6c 65 6f 74 |m is 375| nucleot|
|00005eb0| 69 64 65 73 20 6c 6f 6e | 67 20 61 6e 64 20 68 61 |ides lon|g and ha|
|00005ec0| 73 20 73 69 78 20 6e 75 | 63 6c 65 6f 74 69 64 65 |s six nu|cleotide|
|00005ed0| 20 63 68 61 6e 67 65 73 | 20 69 6e 20 63 6f 6d 70 | changes| in comp|
|00005ee0| 61 72 69 73 6f 6e 20 74 | 6f 20 4d 31 20 52 4e 41 |arison t|o M1 RNA|
|00005ef0| 20 66 72 6f 6d 20 45 73 | 63 68 65 72 69 63 68 69 | from Es|cherichi|
|00005f00| 61 20 63 6f 6c 69 2e 20 | 20 54 68 65 20 72 65 67 |a coli. | The reg|
|00005f10| 69 6f 6e 73 20 66 6f 72 | 20 70 72 6f 6d 6f 74 69 |ions for| promoti|
|00005f20| 6f 6e 20 61 6e 64 20 74 | 65 72 6d 69 6e 61 74 69 |on and t|erminati|
|00005f30| 6f 6e 20 6f 66 20 74 72 | 61 6e 73 63 72 69 70 74 |on of tr|anscript|
|00005f40| 69 6f 6e 20 61 72 65 20 | 63 6c 6f 73 65 6c 79 20 |ion are |closely |
|00005f50| 63 6f 6e 73 65 72 76 65 | 64 2c 20 62 75 74 20 61 |conserve|d, but a|
|00005f60| 64 6a 61 63 65 6e 74 20 | 72 65 67 69 6f 6e 73 20 |djacent |regions |
|00005f70| 6f 66 20 6e 75 63 6c 65 | 6f 74 69 64 65 20 73 65 |of nucle|otide se|
|00005f80| 71 75 65 6e 63 65 73 20 | 73 68 6f 77 20 63 6f 6e |quences |show con|
|00005f90| 73 69 64 65 72 61 62 6c | 65 20 64 72 69 66 74 2e |siderabl|e drift.|
|00005fa0| 00 00 19 54 68 65 20 73 | 65 71 75 65 6e 63 65 20 |...The s|equence |
|00005fb0| 6f 66 20 74 68 65 20 52 | 4e 61 73 65 20 50 20 52 |of the R|Nase P R|
|00005fc0| 4e 41 20 67 65 6e 65 20 | 6f 66 20 53 2e 20 74 79 |NA gene |of S. ty|
|00005fd0| 70 68 69 6d 75 72 69 75 | 6d 20 69 73 3a 0d 0d 61 |phimuriu|m is:..a|
|00005fe0| 67 67 61 61 67 63 67 63 | 61 67 63 67 63 61 67 67 |ggaagcgc|agcgcagg|
|00005ff0| 67 74 67 61 63 61 61 61 | 67 74 63 67 63 67 63 67 |gtgacaaa|gtcgcgcg|
|00006000| 61 61 63 63 63 61 63 74 | 61 74 61 63 74 67 63 67 |aacccact|atactgcg|
|00006010| 63 67 63 63 47 41 41 47 | 43 54 47 41 43 43 41 47 |cgccGAAG|CTGACCAG|
|00006020| 41 43 41 47 54 43 47 43 | 43 47 43 54 54 43 47 54 |ACAGTCGC|CGCTTCGT|
|00006030| 43 47 54 43 47 54 43 43 | 54 54 54 43 47 47 47 47 |CGTCGTCC|TTTCGGGG|
|00006040| 41 47 41 43 47 47 47 43 | 47 47 41 47 47 47 47 41 |AGACGGGC|GGAGGGGA|
|00006050| 47 47 41 41 41 47 54 43 | 43 47 47 47 43 54 43 43 |GGAAAGTC|CGGGCTCC|
|00006060| 41 54 41 47 47 47 43 41 | 47 47 47 54 47 43 43 41 |ATAGGGCA|GGGTGCCA|
|00006070| 47 47 54 41 41 43 47 43 | 43 54 47 47 47 47 47 47 |GGTAACGC|CTGGGGGG|
|00006080| 47 41 41 41 43 43 43 41 | 47 47 41 43 43 41 47 54 |GAAACCCA|GGACCAGT|
|00006090| 47 43 41 41 43 41 47 41 | 47 41 47 43 41 41 41 43 |GCAACAGA|GAGCAAAC|
|000060a0| 43 47 43 43 47 41 54 47 | 47 43 43 43 41 43 47 54 |CGCCGATG|GCCCACGT|
|000060b0| 41 41 47 54 47 47 47 41 | 54 43 41 47 47 54 41 41 |AAGTGGGA|TCAGGTAA|
|000060c0| 47 47 47 54 47 41 41 41 | 47 47 47 54 47 43 47 47 |GGGTGAAA|GGGTGCGG|
|000060d0| 54 41 41 47 41 47 43 47 | 43 41 43 43 47 43 47 43 |TAAGAGCG|CACCGCGC|
|000060e0| 47 47 43 54 47 47 54 41 | 41 43 41 47 54 43 43 47 |GGCTGGTA|ACAGTCCG|
|000060f0| 54 47 47 43 41 43 47 47 | 54 41 41 41 43 54 43 43 |TGGCACGG|TAAACTCC|
|00006100| 41 43 43 43 47 47 41 47 | 43 41 41 47 47 43 43 41 |ACCCGGAG|CAAGGCCA|
|00006110| 41 41 54 41 47 47 47 47 | 54 54 43 41 54 41 41 47 |AATAGGGG|TTCATAAG|
|00006120| 47 54 41 43 47 47 43 43 | 43 47 54 41 43 54 47 41 |GTACGGCC|CGTACTGA|
|00006130| 41 43 43 43 47 47 47 54 | 41 47 47 43 54 47 43 54 |ACCCGGGT|AGGCTGCT|
|00006140| 54 47 41 47 43 43 41 47 | 54 47 41 47 43 47 41 54 |TGAGCCAG|TGAGCGAT|
|00006150| 54 47 43 54 47 47 43 43 | 54 41 47 41 54 47 41 41 |TGCTGGCC|TAGATGAA|
|00006160| 54 47 41 43 54 47 54 43 | 43 41 43 47 41 43 41 47 |TGACTGTC|CACGACAG|
|00006170| 41 41 43 43 43 47 47 43 | 54 54 41 54 43 47 47 54 |AACCCGGC|TTATCGGT|
|00006180| 43 41 47 54 54 54 43 41 | 43 54 54 63 74 74 63 61 |CAGTTTCA|CTTcttca|
|00006190| 74 61 61 61 61 63 63 63 | 67 63 74 74 63 67 67 63 |taaaaccc|gcttcggc|
|000061a0| 67 67 67 74 74 74 74 74 | 67 63 74 74 74 74 61 63 |gggttttt|gcttttac|
|000061b0| 74 67 67 67 63 67 67 63 | 61 67 67 61 74 67 61 61 |tgggcggc|aggatgaa|
|000061c0| 74 67 61 63 74 67 74 63 | 63 61 63 67 61 63 67 63 |tgactgtc|cacgacgc|
|000061d0| 74 61 74 61 74 63 63 61 | 61 61 61 67 61 61 61 67 |tatatcca|aaagaaag|
|000061e0| 63 67 67 63 74 74 61 74 | 63 67 67 74 63 61 67 74 |cggcttat|cggtcagt|
|000061f0| 74 74 63 61 63 63 74 63 | 74 74 63 61 74 61 61 61 |ttcacctc|ttcataaa|
|00006200| 61 63 63 63 67 63 74 74 | 63 67 00 00 00 00 00 00 |acccgctt|cg......|
|00006210| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00006220| 00 00 07 a0 43 41 52 44 | 00 00 37 76 00 00 00 00 |....CARD|..7v....|
|00006230| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 7f 3a |........|.......:|
|00006240| 00 00 0a 96 00 00 00 00 | 00 00 00 00 00 03 00 00 |........|........|
|00006250| 07 56 00 06 42 61 65 72 | 2c 20 4d 2e 20 46 2e 2c |.V..Baer|, M. F.,|
|00006260| 20 52 65 69 6c 6c 79 2c | 20 52 2e 20 4d 2e 2c 20 | Reilly,| R. M., |
|00006270| 4d 63 43 6f 72 6b 6c 65 | 2c 20 47 2e 20 4d 2e 2c |McCorkle|, G. M.,|
|00006280| 20 48 61 69 2c 20 54 2e | 2c 20 41 6c 74 6d 61 6e | Hai, T.|, Altman|
|00006290| 2c 20 53 2e 20 61 6e 64 | 20 52 61 6a 42 68 61 6e |, S. and| RajBhan|
|000062a0| 64 61 72 79 2c 20 55 2e | 20 4c 2e 20 20 31 39 38 |dary, U.| L.  198|
|000062b0| 38 20 20 54 68 65 20 72 | 65 63 6f 67 6e 69 74 69 |8  The r|ecogniti|
|000062c0| 6f 6e 20 62 79 20 52 4e | 61 73 65 20 50 20 6f 66 |on by RN|ase P of|
|000062d0| 20 70 72 65 63 75 72 73 | 6f 72 20 74 52 4e 41 73 | precurs|or tRNAs|
|000062e0| 2e 20 20 4a 2e 20 42 69 | 6f 6c 2e 20 43 68 65 6d |.  J. Bi|ol. Chem|
|000062f0| 2e 20 32 36 33 3a 32 33 | 34 34 2d 32 33 35 31 00 |. 263:23|44-2351.|
|00006300| 00 18 57 65 20 68 61 76 | 65 20 67 65 6e 65 72 61 |..We hav|e genera|
|00006310| 74 65 64 20 6d 75 74 61 | 6e 74 73 20 6f 66 20 4d |ted muta|nts of M|
|00006320| 31 20 52 4e 41 2c 20 74 | 68 65 20 63 61 74 61 6c |1 RNA, t|he catal|
|00006330| 79 74 69 63 20 73 75 62 | 75 6e 69 74 20 6f 66 20 |ytic sub|unit of |
|00006340| 45 73 63 68 65 72 69 63 | 68 69 61 20 63 6f 6c 69 |Escheric|hia coli|
|00006350| 20 52 4e 61 73 65 20 50 | 2c 20 61 6e 64 20 68 61 | RNase P|, and ha|
|00006360| 76 65 20 61 6e 61 6c 79 | 73 65 64 20 74 68 65 69 |ve analy|sed thei|
|00006370| 72 20 70 72 6f 70 65 72 | 74 69 65 73 20 69 6e 20 |r proper|ties in |
|00006380| 76 69 74 72 6f 20 61 6e | 64 20 69 6e 20 76 69 76 |vitro an|d in viv|
|00006390| 6f 2e 20 20 54 68 65 20 | 6d 75 74 61 6e 74 73 2c |o.  The |mutants,|
|000063a0| 20 41 33 33 33 2d 3e 43 | 33 33 33 2c 20 41 33 33 | A333->C|333, A33|
|000063b0| 34 2d 3e 55 33 33 34 2c | 20 61 6e 64 20 41 33 33 |4->U334,| and A33|
|000063c0| 2d 3e 43 33 33 33 20 61 | 72 65 20 77 69 74 68 69 |->C333 a|re withi|
|000063d0| 6e 20 74 68 65 20 73 65 | 71 75 65 6e 63 65 20 55 |n the se|quence U|
|000063e0| 47 41 41 55 20 77 68 69 | 63 68 20 69 73 20 63 6f |GAAU whi|ch is co|
|000063f0| 6d 70 6c 65 6d 65 6e 74 | 61 72 79 20 74 6f 20 74 |mplement|ary to t|
+--------+-------------------------+-------------------------+--------+--------+
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